Isolation of Original Amino Acid, Vitamin and Carbon Source Mutants in the Green Alga Scenedesmus obliquus
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منابع مشابه
Purification and characterization of cytochrome c6 from the unicellular green alga Scenedesmus obliquus.
Purification of a soluble cytochrome c6 from the unicellular green alga Scenedesmus obliquus by a simple and rapid method is described. The purification procedure includes ammonium sulfate precipitation and non-denaturating PAGE. The N-terminal sequence of the first 20 amino acids was determined and shows 85% similarity and 75% identity to the sequence of cytochrome c6 from the green alga Monor...
متن کاملEvidence for nickel in the soluble hydrogenase from the unicellular green alga Scenedesmus obliquus.
Cultures of the green alga Scenedesmus obliquus were grown in the presence of either the chelating reagent EDTA or NiCl2 in various concentrations and assayed for hydrogenase catalyzed photohydrogen evolution after an anaerobic dark adaptation period. Cultivation of algae in the presence of 100 microM EDTA inhibited the formation of hydrogenase activity by 37%. After a cultivation of the cells ...
متن کاملBioenergetic Strategy for the Biodegradation of p-Cresol by the Unicellular Green Alga Scenedesmus obliquus
Cultures from the unicellular green alga Scenedesmus obliquus biodegrade the toxic p-cresol (4-methylphenol) and use it as alternative carbon/energy source. The biodegradation procedure of p-cresol seems to be a two-step process. HPLC analyses indicate that the split of the methyl group (first step) that is possibly converted to methanol (increased methanol concentration in the growth medium), ...
متن کاملDifferential Changes in the Photosynthetic Pigments and Polyamine Content during Photoadaptation and Photoinhibition in the Unicellular Green Alga Scenedesmus obliquus
Polyamines, Photosynthetic Apparatus, Photoinhibition, Photoadaptation, Scenedesmus obliquus In the unicellular green alga Scenedesmus obliquus the level of photoinhibition and the recovery of the cells after reversal to the initial light conditions in relation to the pre-photoadaptation of the culture to low, medium and high light intensity was determined. The changes in the photosynthetic pig...
متن کاملTransfer ribonucleic acid from Scenedesmus obliquus D3.
Scenedesmus obliquus is a green alga whose cells have a well-defined nucleus, and contain a chloroplast. Even when grown in the dark these cells retain their chloroplast. Because of the toughness of the cell wall, extraction of tRNA requires either vigorous homogenization of the cells before phenol extraction or prolonged phenol extraction at 60°C. Determination of the amount of tRNA and its pu...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1980
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-119-1-273